bacterial generations - how are they tracked ?
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bacterial generations - how are they tracked ?         


Author: marks542004
Date: Sep 8, 2008 11:10

Hi all,

I have been reading a paper by Richared Lenski on a long running
experiment using e coli bacteria.
He says that they have taken a sample and frozen it every 500
generations.
Is that meant literally or an approximation and how do you tell ?

lots of Detail not really required , I dont have the background :( ,
just curious

I found the paper referred to at Wikipedia and then Conservipedia (?)
which seems to be a bit of a whackjob site

regards
2 Comments
Re: bacterial generations - how are they tracked ?         


Author: DK
Date: Sep 11, 2008 09:28

In article darwin.ediacara.org>, marks542004@yahoo.com wrote:
>Hi all,
>
>I have been reading a paper by Richared Lenski on a long running
>experiment using e coli bacteria.
>He says that they have taken a sample and frozen it every 500
>generations.
>Is that meant literally or an approximation and how do you tell ?

Close approximation. The assumption is that no bacteria die.
Cells can be counted. Every doubling of cell number is then one
generation.

DK
no comments
Re: bacterial generations - how are they tracked ?         


Author: Joachim Pimiskern
Date: Sep 11, 2008 09:28

marks542004@yahoo.com schrieb:
> I have been reading a paper by Richared Lenski on a long running
> experiment using e coli bacteria. He says that they have taken
> a sample and frozen it every 500 generations.
> Is that meant literally or an approximation and how do you tell ?

There seems to be a slight difference between the two
daughter cells of a single celled organism:

http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal...
http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0030224

Regards,
Joachim
no comments

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