Hi everybody
Myself a researcher working in India..
Can anybody please provide me with S pombe Host n its relevant expression
vector system for expression of Human parathyroid hormone...
Due regards
nipun

Hi everybody
Myself a researcher working in India..
Can anybody please provide me with S pombe Host n its relevant expression vector system for expression of Human parathyroid hormone...
Due regards
nipun 

TWO Postdoctoral Fellowship Positions available
Biology of RNA Metabolism in Eukaryotes
National Institutes of Health (NIH), Bethesda, Maryland

The Fellows will investigate molecular mechanisms involved in RNA
metabolism (this may include links between transcription, RNA
processing, nuclear transport, and translation). Fission yeast serve
as a model system to study human as well as yeast factors. We also
use in vitro biochemistry, mammalian tissue culture and genetically
altered mice. Laboratory approaches integrate cell biology,
molecular biology, genetics, biochemistry and structural biology.

Dear all,

I have noticed that in some cases when S. cerevisae is grown in shaker
flasks in order to express membrane proteins the shaking is done at
quite slow speed (130-160 rpm). Has anyone any kind of clue why it is
so ?

Could it have something to do with inhibiting the formation of foam ?
If so does anyone know if antifoaming agents are detrimental for
membrane protein production.

Thanks already beforehand.

-Juho

Dear All,

Platforms for gene expression microarray have evolved over the time. One consequence of this "evolution" is that the number of genes across different platforms varies along with other changes. This situation makes it difficult to cluster array data obtained from different platforms.

I wonder if anyone could suggest way or softwares etc to pre-process and cluster gene expression data across different platform and/or different versions of the same platform. I would appreciate any help with this.

Shaun

________________________________

From: yeast-bounces@oat.bio.indiana.edu on behalf of yeast-request@oat.bio.indiana.edu
Sent: Wed 20/08/2008 3:04 AM
To: yeast@magpie.bio.indiana.edu
Subject: Yeast Digest, Vol 39, Issue 7

Send Yeast mailing list submissions to
yeast@net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
http://www.bio.net/biomail/listinfo/yeast
or, via email, send a message with subject or body 'help' to
yeast-request@net.bio.net

You can reach the person managing the list at
yeast-owner@net.bio.net

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Yeast digest..."

Hi,

I have been having problems with my 5-FOA Selection.

I am trying to knock out a section of a gene in S.cerevisiae through a
pop-in/pop-out method. I initially inserted an Ura3 gene into the region I
wanted to delete and made the construct (I) with the URA3 gene. This
construct (I) was made on a background strain BY4741 clean del Ura3Knockout
replaced with a Kan marker (on a BY4741 WT).

I then used this strain to have my clean knockouts by transforming PCR
product homologous to the flanking region of the Ura3 gene that was inserted
through the FOA selection method. This way I hoped to achieve a clean
Knockout- Construct(II). But unfortunately, I could not get the second stage
of FOA selection to work.

Hi,
ATCC 208282 or 208286 is likely something interesting. These yeast
strains were made form Liz Jones's lab long ago.
hope it helps.

Jim Zhou, Ph. D.
Sr. Collection & Research Scientist
Head, Mycology Program
American Type Culture Collection (ATCC)
10801 University Blvd
Manassas, VA 20110
Tel. 703-365-2761 (office)
Fax. 703-365-2770
A Global Biological Resource Center

-----Original Message-----
From: yeast-bounces@oat.bio.indiana.edu
[mailto:yeast-bounces@oat.bio.indiana.edu] On Behalf Of Arthur Lustig
Sent: Tuesday, August 19, 2008 10:15 AM
To: yeast@magpie.bio.indiana.edu
Subject: [Yeast] PEP4 deletion plasmid

I am looking for a pep4 deletion plasmid other than a kanr form. Liz Jones
would have been the obvious source, but with her passing I am not sure of a
good source. I would appreciate any help in this matter.
Art Lustig

Arthur J. Lustig PhD
Professor, Department of Biochemistry
Tulane University Health Sciences Center
1430 Tulane Avenue
New Orleans, LA 70112
ph:504-988-3688
fax:504-988-3687

PEP4 deletion plasmid

I am looking for a pep4 deletion plasmid other than a kanr form.  Liz Jones would have been the obvious source, but with her passing I am not sure of a good source.  I would appreciate any help in this matter.
Art Lustig

Hi all,

I'm looking to extract naturally occurring 2 micron plasmid from yeast.
There seem to be a range of plasmid DNA preps but having tried the qiagen
plasmid kit and failed i would appreciate a recommendation before buying
into different kits.

Does anyone have any experience with this?

All help much appreciated

Ellie

PS. thanks for all replies regarding aAA selection- please feel free to
contact me if interested in the results!

Hi all

I am currently working with the yeast strain YBZ-1 and I have
transformed it with several pACT2 -based plasmids (each one
individually). The transformations went fine and I had colonies only on
the plates...

Thanks to Charlie, Brenda and everyone for a great meeting in Toronto
last month and for doing all the hard work to make it such a success.
Harry and I had a very good time.

Best wishes,

Carl

Carl Singer | Managing Director

SingerInstrument Co. Ltd.

www.singerinstruments.co.uk
<blocked::http://www.singerinstruments.co.uk/>

yeast@singerinst.co.uk singerinst.co.uk>

T: +44 (0)1984 640226

F: +44 (0)1984 641166

Singer Instruments.... A responsibility to science