Hi all, is anyone know where I could retrieve the members of each Uniref
cluster. The fasta file I downloaded from uniprot ftp site,
uniref90.fasta, includes only the Uniref cluster name and the representative
sequence information but does not include which sequences belong the this
cluster.

thanks,
oznur

Dear Ashley,
I am completing my PhD on production, characterization and application of alkaline phosphatase. However my HPLC experiments are still pending. As for GFC, i got quite good results and consitant activity.
For further discussion, we can communicate through email.
Regards

Bashir Ahmad
Ph.D Research Scholar
Deptt.of Microbiology&Biotechnology
Quaid i Azam University, Islamabad 45320
Pakistan.
mbashir@bs.qau.edu.pk

Visiting Research Fellow
Uni of Waterloo, Ontario,Canada
b2ahmed@scimail.uwaterloo.ca

While I was searching for low glycemic index breads I came across a complex
oligosaccharide and an alpha-glucosidase inhibitor called Acarbose
(GlucobayR). Evidently used to lower the glycemic index of white bread right
down to the level of a carrot (17). The GI of normal white bread is 70. Low
glycemic index foods keep serum glucose levels and insulin levels down. High
serum glucose, insulin and insulin like growth factor can promote the growth
of tumors

Acarbose is an alpha-glucosidase inhibitor and doesn't inhibit the beta
enzyme but it has analogs that do.

It also reminded me of amygdalin which releases cyanide when encountered by
the enzyme beta-glucosidase. I thought if there is a safe beta-glucosidase
inhibitor that works in serum and normal cells and fails in cancer cells for
whatever reason then amygdalin may potentially be used orally (assuming that
it can be taken up by cancer cells). Just a thought. I think amygdalin is
currently only useful palliatively and is injected into tumors. It was
recently approved here in Australia on compassionate grounds.

Anyone who wants to rush off and patent this idea please let me be the first
lab rat :)

Hi everyone,
I have been using an osmotic shock (20%% sucrose and then water - taken from
"current protocols" or the pET manual) - based protocol, for the extraction
of a protein expressed with pET26b (periplasmic exp. with a pelB leader
sequence).
It looks fine on the small scale (>50ml culture), but I'm having trouble
up-scaling it to ~1L.
does anyone know a protocol designed for large scale periplasmic preparation
(non-danaturing).
cheers,

Dr. Balaal,

I hope you do not mind for me contact you directly. I was searching on
the internet for "endotoxin removal" methods and came across your
discussion thread on www.indusbio.co.in <http://www.indusbio.co.in/> .
Do you know if the charged filter capsules (e.g. Pall, Millipore) that
you mentioned, or any others that may be available, would be
compatible/effective with a solution of a compound in an organic solvent
(e.g. ethanol) from which we would wish to purge endotoxin, versus
aqueous solutions?

Thanks in advance for your advice - I appreciate it,

Erik

Erik Flahive

Pfizer Global R&D - La Jolla Labs

10646 Science Center Drive

San Diego, CA 92121

(858)-622-7541

How about the NCBI keywords?

Hi, is anyone aware of a database which provides functional protein
classification in broad terms, for example "kinases", "receptors" would be
two protein classes. I've just checked the root terms of InterPro, it was
still too specific for my task.

thanks
-
oznur

Hi all,

In an effort to help new PIs at smaller
research institutes form collaborations, I’ve made a Google Maps mashup
designed for people to add push-pins for any specialist equipment they might be
willing to share/collaborate with. It’s pretty basic at the moment, and
currently covers only a small range of equipment that I’ve needed for my own research
projects (however, I am happy to extend this much further).

http://www.pageforaday.com/xtalwizard/maps/

To circumvent data protection issues, people who list equipment need only
submit a webpage which includes suitable contact information rather than
submitting a contact name/e-mail address/phone number.

I’m not sure if something like it already exists (please let me know if it
does), but if people find it useful I will try to fix any bugs and improve it
with any user suggestions.

Thanks,

AGS

_________________________________________________________________
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hello to all,
i am new in the field and trying to run my sample in GC. i have
extracted my oil from seed in petroleum ether using soxhlet apparatus.
to transesterifying it it is treated with methanolic KOH. the problem
starts now as i don't know that how to prepare the standard sample. i
have standards of linoleic acid and oleic acid from sigma aldrich. i
don't know the exact procedure to prepare these standards so as to run
into GC. can it be made by simply dissolving in methanol

this is very bad that ads are taking place in this place of discussion