dk@no.email.thankstospam.net (DK) wrote in
news:JGTGn.9461$TL5.1130@newsfe24.iad:

Meanwhile, I have assayed the HRP-labeled antibody, it works fine,
supposedly my periodate is ok.

I just wonder how even such a renowned source like the book by Harlow
and Lane (Antibodies: A Laboratory Manual) can have such erroneous
information.

Conclusion: Don't trust blindly any source. Reminds me a bit on the
spinach issue that probably many of us had to pay for by being forced
to eat that 'so healthy' veggie -because of its 'high' iron content -
over and over again in our childhood.

Wo

Hello sir

My name is Gavin Clements from new Zealand and I was wondering if you had the number for the people who manufacture these syringes as we are looking for them as well . If you do could you pass this information on to us .
Many Thanks Gavin

+64 21 163 7221

One wonders whatever happened to the prices on Gene Synthesis.. they haven't
changed in about a year I'd say, is that right? Is this the beginning of
price fixing, a technological limitation, or am I imagining the whole
situation?
-Cathal

 Hi!
I want to correct DK's point. If it is proofreading polymerase, 3'A will get depleted off as a result of proof reading. Taq Pol/ Long Pol amplified products can generally be used for TA cloning. Their fact will have that info.

Secondly, to me the suggestion to Point 2 is tedious.
Gel purified PCR product can be directly quantified and ligation reaction can be set up, depending upon the size of the insert, one needs to clone. 5:1::insert:vector is the optimal molar ratio for cloning insert of 1250bp. Just quantify the insert and go ahead.
All the best.

Shifali

On Thu, 25 Dec 2008 Shri wrote :

Hi all members,
I have amplified PCR product of 1250 bp from Genomic DNA
my question is
1 Whether it can be cloned in TA cloning vector the required size.
2. how to calculate the concentration of the PCR product after the
reaction

hi can anyone help me how to use neural network in matlab? like how to
give inputs,weights,targets.?

Hi does anyone know how PEG can be used for cell lysis.? whats the
mechanism of cell lysis by PEG?

Dear Friends,

We are planning to set up a molecular biology laboratory from scratch. I am
required to make a list of equipments required for regular use in the
laboratory. We have already shortlisted major equipment like

chemidoc system, RT PCR, 2D gel electrophoresis system, laminar air flow,
etc

with smaller equipments like submarine gel electrophoresis units, PAGE
units, western and southern blot apparatus, speed vac, hand held uv torch,
uv transilluminator, tabletop centrifuge, autoclave,

and other everyday use apparetus like waterbath, pH meter, magnetic
stirrers, vortex, incubator, oven, digital balance and the freezers of -20
and -70 degrees Celsius etc

I need some inputs on whether there is anything crucial from the point of
view of a mole biol lab that I may have missed out... Any and all
suggestions are welcome!

Regards,
--Yg

Dear experts,

I know this post might be considered a bit off topic for this NG, but
as I feel quite a bit like home here, I dare to start my research
here.

I am in charge of setting up a laboratory data management system
(LIMS) for a new lab. From hard- and software, I may quite freely
choose while it should not be too expensive in the beginning. I am
aware that there is a lot of possible solutions on the market, but yet
I have no real good point where I could start from. When feasible, I'd
prefer open source.

That's why I'd like to ask you for some guidance, please!

The desired system needs to integrate people (concepts, lab notebooks,
reports, references, secure (encrypted) email) with SOPs, equipment
(data, status, alarms) and should smoothly integrate into a server
based network including access from remote locations. It should be
scalable (we start with a small lab and 5 people) and be expandable to
manage stock and manufacturing later, meaning, it must be able to grow
with a company. Of course the data management must fulfill the
requirements for patent applications etc.