is fish immunology system a good model for human immunology system? Dear sir
I need detais of it

Shyam Narayan Labh
Senior Lecturer, TU Kath. Nepal
Research Scholar (SAARC Fellow)
Aqua Reserach Lab
Department of Zoology
University of Delhi
Delhi 110007
India
Email Address: snlabh@yahoo.com
Web site: www.aquaresearchlab.org
http://www.tagged.com/snlabh

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Hello,
My question is not completely related to immunology protocols, however
it does have something to do with the way we analyze data.
I have data from studies were I monitor NOD mice for diabetes
development, when given 2 different treatments. I have plotted the
data using Prism and the Survival Curve function, and proceeded to do
a Log-Rank Analysis.
My questions/Concers are as follows:
(1) what does the long-rank mean? does it only refer to the AGE that
mice became diabetic?
(2) what test should i use to check whether the incidence of diabetes
at the end of the experiment was significantly different? i.e. i dont
care if my mice starting getting diabetic at an early age, all i want
to know is at the end of the treatment, i had these many mice that
were diabetic(and so many that were not...

Hello,

I am a recent graduate with a degree in Honour biology (specialize in
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I have been using the mouse macrophage cell line J 774 for the analysis of
intracellular activity of anti bacterials against S aureus. I am using RPMI
with 10%% FBS for the cell growth. I observe a change in the morphology of
the cell line as we passage them in vitro. They are spindle shaped to
circular to begin with, but become highly differentiated with star shaped
apperance with multiple granules. Is it a normal phenotype? Can anyone
tell me the exact morphology of these cells? and also the difference
between j774.1 and j774.2 cells
thanks
lakshmi

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Dear Stacy:

I have a guestion about protein concentrator. Would you please do me a favor?

I concentrate protein in dilute solution by putting it in a dialysis
bag(Cellu.Sep.H1 MWCO 5000),sprinkle AQUACIDE III over the bag, and
leave it overnight at 4 degrees. Next morning, I received the clear
solution and precipitate and both of them were no signals on SDS-PAGE.
Both of them had UV absorption spectra at wavelenth 280nm. I also
dissolved Aquacide III which is outside the bag in water and it had UV
absorption spectra at wavelenth 280nm. Could Aquacide III outside the
bag pass through the membrane? If it could pass through the membrane,
what's wrong with my procedures? And how to improve it?

Thanks for your helps
Regards,
National Taiwan University,
Chemistry Department
Shang-Jen Wu

If you want to avoid this IgG aggregation, keep the pH at 4.5+ 0.25, and
dialyze it Vs 0.3M Glycin

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Dear Sir
My question is as follow

For a antigen with discontinuous epitope, its digested into peptides in endosome,
then whether the peptide(discontinuous epitope) is binded with several peptides?
finline

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