Hi,

I have an odd problem with a secretory IgA ELISA we are trying to get
working. We are using a monoclonal capture antibody with a
polyclonal conjugate and TMB as the substrate. If we plate a
constant...

The distance learning course 'Introduction to Bioinformatics' from the
University of Manchester, UK, begins again on 6 October 2008. This is a
Masters level course; credits can count towards either the MSc in
Bioinformatics or the MSc in Immunology and Immunogenetics
(http://www.ls.manchester.ac.uk/postgraduate/distancelearning/).

The course is delivered in a Virtual Learning Environment, which allows
us to extend the classroom into the web. Teaching and learning are
focussed around tutor-supported exercises. In this course, participants
work together on the interpretation of their results, before receiving
feedback from the course tutor. Participants who wish to be assessed
for credits at MSc level should complete additional independent research.

Week 1 Introduction to distance learning
Bioinformatics as a knowledge-based discipline

Weeks 2 and 3 Introduction to the sequence databases
Quality, redundancy and annotation
Sequence retrieval exercise and group discussion

Acapulco/Mexico & Hawaii/USA 2009 Immunology Conferences

CALL FOR ABSTRACTS / PAPERS / POSTERS:

The Frontiers in Immunology Research 2009 Conferences <www.firnweb.com>
will be held in Acapulco, Mexico (January 8-11 at the Hyatt Regency
Hotel) and in Hawaii (Kona), USA (July 22-26 at the Sheraton Hotel.) You
may participate as panel organizer, presenter of one or two papers or
posters or as an observer.

The deadlines for abstract / poster submissions and registration are:
September 30th, 2008 (Acapulco) and
March 30th, 2009 (Hawaii).

For more information, please contact the Frontiers in Immunology
Research Network (FIRN) as follows:
FIRN, 64 Holden Street, Worcester, MA 01605-3109, USA.
Telephone: 508-852-3937, Fax: 508-595-0089, Email: firnweb.com>

The EMD Serono Phage Technology Group is a multi-disciplinary team
interacting with Oncology disease researchers in USA and Europe. Our
Group is responsible for developing therapeutic antibodies to novel
targets for use in the treatment of Cancer.
We are seeking a highly motivated Research Scientist/Senior Scientist
who will be responsible for the selection of functional antibodies
using phage display technology. This will include developing selection
strategies, performing phage selection, preparing samples for DNA
sequencing, analyzing HT sequence results, and cloning and expressing
the selected candidates. Other responsibilities include the
construction of affinity maturation libraries as well as the
development and running of screening assays.
Strong phage display experience and molecular biology background
required, and yeast display experience preferred.
The successful candidate will be able to work effectively with minimal
supervision required on established assignments, while exercising
total independence and technical discretion in design and execution of
projects and interpretation of results.
The successful candidate must have a Ph.D. with strong experience in
phage display technology (3+years) and molecular biology. Industry ...

Hi,
I want to do immunohistochemistry detecting two targets at the same
time. For various reasons I am going to use AEC as substrate for HRP
and BCIP/NBT as AP substrate. Now I am looking for a good
counterstain. Because of the AEC the counterstain has to work with an
aqueous embedding medium.
Has anybody a good idea? Thank you very much for your help in advance,
Sven

I have been conducting research on the effects of phytochemicals and
cell cycle and apoptosis and I would like to find a way to run both
assays on the same cells.

I am currently using the NIEHS protocol for measuring cell cycle with PI
and an anti-BrdU FITC antibody. I would like to keep this method since I
really like the added confidence of S phase determination that the BrdU
tagging gives me. I am looking for an apoptosis marker to add to this,
does anyone have anything they know or would hypothesize would work? I'm
thinking either a caspase substrate or some sort of antibody to an
apoptosis-related protein.

My current protocol gives me excellent cell cycle staining and it
involves:

1. Adding BrdU to the cells for 40 minutes
2. Fixing the cells with ethanol
3. Solubilizing the cells with 2N HCl + 0.5%% Triton-X 100
4. Overnight staining with the anti-BrdU antibody (BD)
5. Overnight staining with PI

I then read on the flow with a FACSCalibur (FL1 = FITC, FL3 = PI).

Is it possible for you to let me have any information that you have for
latex allergy up to 1996. I would really like 1995 and before if that is
possible.
If you can't provide can you please tell we where I could find this
information and if it was availble to the Australian Health Department.
I know I am being painful and I am sorry, but I just don't know where
the accurate information is.
Many thanks,
Chris Diment

---------------------------------------------------------------------------------------------

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Hello,

I would appreciate receiving comments about the detachment procedure for
cultured Mesenchymal stem/stromal cells (MSCs) for FACS analysis.

I am starting immunophenotyping these cells and I am concerned about
damaging surface antigens using trypsin; however, trypsin is the only
procedure that is working in my hands right now:

other methods I tried:

EDTA and a Cell dissociation buffer (Enzime free, PBS based) from Gibco;
these last two methods were not succesful since the cells formed very
tight clumps which did not come out with filtering methods, and lots of
the cells died.

So I am using trypsin at a low concentration ( 0.05%%), and let the cells
stand in medium with serum for one hour to let them recover from the
trypsinization process.

Do you have a detailed procedure for this, with particular emphasis on
(1) trypsin-EDTA concentration (2) time left between trypsinization and
recovery of any damaged surface antigen until FACS analysis.

Any thoughts are welcomed

Thank you in advance.

Hi,

Do both extracts, water and alcoholic.

lit

--- On Sun, 29/6/08, immuno-request@oat.bio.indiana.edu oat.bio.indiana.edu> wrote:
From: immuno-request@oat.bio.indiana.edu oat.bio.indiana.edu>
Subject: Immuno Digest, Vol 34, Issue 2
To: immuno@magpie.bio.indiana.edu
Date: Sunday, 29 June, 2008, 1:05 AM

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Today's Topics:

I HAVE A FUNDAMENTAL QUERY REGARDING MY PROJECT [ immunomodulatory
action of aloe vera and andrographis paniculata in human pbmcs].
a] what extract[ alcoholic or aqueous] of the above mentioned plant
extracts should i use??
awaiting your reply.......................ASHIS