Dear all,

I am trying to rescue the T-DNA insertion and try to know the sequence which is flanking the T-DNA left or right border in the plant transformants. The transformed plants have a big genome. Now I try to find a very useful method to know the insertion site in the plant genome.

Do you know any methods or have any experiences or suggestions in how to rescue / recover the T-DNA insertion? Could you let me know?

please email me: xwwang@uga.edu

Thanks

xuewen