http://www.nnseek.com/e/bionet.genome.arabidopsis/ Posts for bionet.genome.arabidopsis Mon, 22 Sep 2008 14:39:31 PDT http://www.nnseek.com/ http://www.nnseek.com/img/64.png 64 64 NNSeek http://www.nnseek.com/e/bionet.genome.arabidopsis/sourcing_fm4_64_102595570t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/sourcing_fm4_64_102595570t.html
I am looking into buying FM4-64 to stain cell membranes in living
Arabidopsis shoots. I see that i can buy 1 mg of FM4-64 from
Invitrogen/Molecular Probes for about $400 (CAD.) I've also found a
second supplier, Calbiochem, that sells FM4-64 under the name
SynaptoRed for $240 (CAD) for 5 mg. The publications i've looked at
have all used FM4-64 from Molecular Probes. I'm wondering if anyone
has used Calbiochem's SynaptoRed/FM4-64 in Arabidopsis, and if they
could comment on it's efficacy at staining cell membranes.

Thanks very much,

danielle

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]]> Mon, 22 Sep 2008 14:39:31 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/post_doctoral_positions_in_plant_molecular_biology_102437874t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/post_doctoral_positions_in_plant_molecular_biology_102437874t.html MELBOURNE AUSTRALIA



Expressions of interest are sought to fill postdoctoral positions within the
Plant Molecular Biology and Biotechnology group, at the Melbourne School of
Land and Environment at The University of Melbourne, highest ranked Research
University in Australia. We are interested in recruiting highly motivated
individuals with a desire to succeed and contribute to the internationally
competitive research activities of the laboratory focussing on molecular
mechanisms of differentiation and cell specific gene regulation during
meristem development, floral transition and reproductive differentiation. A
brief description of research projects is provided below.

MicroRNAs and Epigenetic Regulation of Meristem Development

MicroRNAs (miRNAs) are small non-coding RNAs that regulate expression of
many genes. We have recently discovered a network of shoot apical meristem
specific miRNAs. These include novel plant miRNAs being reported for the
first time. The postdoctoral research project will involve investigation of
role of these novel miRNAs in meristem development and floral transition
using computational and experimental tools. The experimental approach will
include expression profiling of individual miRNAs through gel blots,
microarrays, in situ localizations combined with gain-and loss-of-function
studies in transgenic plants.

Molecular Control of Stem Cell Maintenance in Plants

Shoot apical meristems (SAMs) provide niches for self-sustaining groups of
stem cells that play a critical role in plant development being founder
cells of all aerial plant parts. Maintenance of these stem cells is thus
essential for repetitive organ initiation throughout plant lifecycle. Using
high throughput gene expression profiling technologies we have identified
subset of novel genes that are differentially expressed in shoot apical
meristem of pea and soybeans. The postdoctoral appointee will participate
in a project addressing developmental role of these genes. The approaches
will include laser capture microdissection, mutant analyses, in situ
hybridisations, and reporter gene expression and gain-and loss-of-function
studies in transgenic plants.

Male Germ Line Development and Fertilization in Flowering Plants

Unlike animals where germ line cells diverge from somatic cells during early
embryo development the plant germ lineages initiate late in development at
flowering stage. The male germline in plants begins with an asymmetric
division, after which one of the resulting cells, the generative cell,
divides symmetrically to produce two sperm cells. Research from our
laboratory has revealed that the male germ line cells show transcriptional
repertoire that is highly divergent from other reproductive and somatic
cells. The post-doctoral appointee will participate in a project focusing
on role of cell specific genes and nature of associated regulatory
mechanisms. Potential approaches include Potential approaches include
DNA-protein interaction analyses, chromatin immunoprecipitation (ChiP), gene
knockout in transgenic plants, proteome and methylome analysis,
live imaging of fertilization events.

Applicants should possess a PhD, together with relevant experience in one or
more fields of functional genomics, molecular genetics and cell biology and
be able to provide evidence of proven research productivity in the form of
publications in refereed journals. Candidates should also be experienced in
general molecular biology techniques and ideally have expertise in one or
more of following techniques/approaches; protein-DNA interactions,
protein-protein interactions, ChIP, protein biochemistry and bioinformatics.

The positions are being offered at Research Fellow ( Level A) position with
salary range of $63,451-$68,109 p.a. Candidates with exceptional
achievements can be considered for appointment at a higher salary level.
Applications close on 17 th October 2008. For further details contact:
Professor Prem L Bhalla, tel. +61 3 8344 9651, email premlb@unimelb.edu.au
or Professor Mohan B Singh, tel. +61 3 8344 5051, email mohan@unimelb.edu.au

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]]> Sun, 21 Sep 2008 06:57:50 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/post_doc_position_in_cell_biology_at_ijpb_versailles_102029554t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/post_doc_position_in_cell_biology_at_ijpb_versailles_102029554t.html IJPB, Versailles, France

Recent results obtained in our group on the characterization of the
TON1, FASS/TON2 proteins and their partners uncovered converging
evidences pointing towards evolutionary conservation of some
centrosomal components at the plant cell's cortex. TON1 and FASS
themselves are related to proteins recently shown to localize at the
centrosome in animal cells. TON1 interacts with centrin, a key
component of eucaryotic MTOCs. Moreover, several TON1 and FASS
interactants belong to a new family of cytoskeleton-associated
proteins called TIM which are reminiscent of some animal centrosomal
proteins. These are the first indications ever for a functional and
evolutionary link between animal centrosomes and the cortical
cytoskeleton of plants, apart from gamma-tubulin dependent nucleation.
Through a collaboration between our group and the group of Marilyn
Vantard the successful candidate will work on the functional
characterization of TON1 and TIM proteins in plant cells, and of their
other interacting partners in the cell from a cytological, molecular
genetics and biochemical point of view.

The position is available for candidates with outstanding academic
record and excellent experimental and communication skills. It is
located in Versailles (France) for 30 months, starting January 2009.
Part of the work will be done in Grenoble, in Marilyn Vantard's group.
Salary is 2200-2500 euros per month.

Requirements : Candidates must have a PhD with an excellent academic
record. We are seeking individuals with a strong background in
genetics, molecular biology, biochemistry and microscopy. Experience
in protein biochemistry and/or with fluorescent protein detection and
confocal laser scanning microscopy is a plus. Experience in plant
biology and knowledge of the French language are not mandatory.

Application : Please send cover letter, CV, publication reprints and
names and e-mail addresses of three references to David Bouchez (bouchez@versailles.inra.fr
). The successful candidate will start in January 2009 or soon after.

Relevant publications :
Azimzadeh et al (2008). Arabidopsis TONNEAU1 proteins are essential
for preprophase band formation and interact with centrin. Plant Cell,
in press
Pastuglia & Bouchez (2007). Molecular encounters at microtubule ends
in the plant cell cortex. Curr Op Plant Biol 10:1-7
Pastuglia et al (2006). Gamma-tubulin is essential for microtubule
organization and development in Arabidopsis. Plant Cell 18 : 1412-1425
Camilleri et al (2002). The Arabidopsis TONNEAU2 gene encodes a
putative novel PP2A regulatory subunit essential for the control of
cortical cytoskeleton. Plant Cell, 14 : 833-845.

The Jean-Pierre Bourgin Institute hosts 25 research groups devoted to
plant biology, totalizing 330 people, comprised of 230 permanent staff
and 100 post-docs, PhD students and others students. The IJPB has a
long-standing and respected expertise in the field of plant biology,
including genomics, genetics, breeding, metabolism, cell biology and
development. All tools and resources relevant for integrative plant
biology are available on site. Local facilities in Versailles include
outstanding experimental facilities dedicated to plant culture (4500
m2 glasshouses and 500 m2 controlled environment rooms), state of the
art technical platforms for Microscopy and Cytology (4 confocal
microscopes, classical microscopy, etc), Protein Biochemistry
(electrophoresis, chromatography, MALDI-TOF MS…), Chemistry (FT-IR,
several mass spec including GC-TOF and LC-MS/MS…), and Bioinformatics.

Further details and information : bouchez@versailles.inra.fr


David Bouchez
Institut Jean-Pierre Bourgin
Station de Génétique et Amélioration des Plantes
INRA Centre de Versailles - 78026 Versailles Cedex France
tel : +33 (0)1 30 83 33 94 - fax : +33 (0)1 30 83 33 19
e-mail : bouchez@versailles.inra.fr
http://www-ijpb.versailles.inra.fr

cytoskeleton group :
http://www-ijpb.versailles.inra.fr/en/sgap/equipes/cyto/

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]]> Wed, 17 Sep 2008 06:06:29 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/arabidopsis_conference_survey_summary_101437426t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/arabidopsis_conference_survey_summary_101437426t.html If you are interested in seeing a summary of responses to the Arabidopsis
conference survey please see:
http://www.arabidopsis.org/portals/masc/journal.jsp#survey08

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]]> Thu, 11 Sep 2008 17:36:02 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/_9th_ipmb_congress_st_louis_mo_oct_25_30_2009_101436914t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/_9th_ipmb_congress_st_louis_mo_oct_25_30_2009_101436914t.html
You are invited to attend the 9th International Congress of
Plant Molecular Biology (IPMB), October 25-30, 2009 taking place
in St. Louis, Missouri. Please look at the following website for
all necessary information on this Congress which is composed of
53 symposia and 9 Plenary Lectures on all aspects of plant
molecular biology.

http://www.ipmb2009.org

Sincerely,

Perry Gustafson - Chair IPMB 2009
--------------------------------------------------------------------------
9th International Congress of Plant Molecular Biology, October 25-30, 2009
--------------------------------------------------------------------------
[Apologies, the URL sent from a previous message has been corrected here.]

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]]> Thu, 11 Sep 2008 11:41:03 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/methods_for_rescue_t_dna_in_transformants_101437682t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/methods_for_rescue_t_dna_in_transformants_101437682t.html
I am trying to rescue the T-DNA insertion and try to know the sequence which is flanking the T-DNA left or right border in the plant transformants. The transformed plants have a big genome. Now I try to find a very useful method to know the insertion site in the plant genome.

Do you know any methods or have any experiences or suggestions in how to rescue / recover the T-DNA insertion? Could you let me know?

please email me: xwwang@uga.edu

Thanks

xuewen

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]]> Thu, 11 Sep 2008 08:12:56 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/bioinformatics_analyst_position_101436658t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/bioinformatics_analyst_position_101436658t.html organism. You might be interested in this.



Bioinformatics Analyst

Requisition Code:

PLAN688

Location:

Rockville, Maryland

Department:

Plant Genomics

Schedule:

Full Time





Job Responsibilities: The Bioinformatics Analyst will work on the
curation and annotation of the M. truncatula genome sequence.
Responsibilities will include design and maintenance of web pages
providing access to Medicago genome sequence and annotation and
associated data, including outreach to the users through a community
annotation portal. The ideal candidate will have hands-on research
experience in Medicago genomics and demonstrated competence in
bioinformatics including Perl, SQL, HTML and cgi-scripting.

Qualifications: Successful candidates will have a Bachelor's or Master's
degree in the Biological Sciences, plus experience working with
computers in the field of biological sciences and a familiarity with DNA
sequence and annotation tools and practices. Candidates will also have a
demonstrated competence in appropriate computer languages including
Perl, SQL, HTML and cgi-scripting; web technologies including HTML,
DHTML, XML, CSS; Graphics tools including Photoshop, Fireworks or
similar; Operating Systems including UNIX and UNIX variants, Windows,
and/or Macintosh; Relational Database including knowledge of Sybase and
SQL. The Bioinformatics Analyst will have a solid knowledge of molecular
biology as it relates to DNA sequences, sequencing technologies and
genomics. Strong written and verbal communication skills, excellent
organization and time management skills, ability to meet schedules, the
ability to collaborate across a multi-disciplinary team, and work
independently is a must.



You can apply through the JCVI web site; the position is listed under
Plant Genomics.

https://careers.jcvi.org/careers/



___________________________

Chris Town

Investigator

The J. Craig Venter Institute

9704 Medical Center Drive

Rockville, MD 20850

Office Phone: 301-795-7523

Fax: 301-838-0208

Home Phone: 301-990-0878

Cell Phone: 301-204-6300

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]]> Thu, 11 Sep 2008 02:43:55 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/problem_in_pcr_101260786t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/problem_in_pcr_101260786t.html assisted selection) . i have did the DNA isolation from
green house plant and open field plant. and complete the pcr for diffent
resistant gene. for near about 1500 sample the pcr is working fine got the
good amplification, but when i have again collected the samples after 30 day
the DNA Quality looks good on gel ,but not get amplified in pcr reaction. we
are tried the things but its not working. i would be thankful if some one
suggest me to over come the problem.

lookinforward for suggestion


With Regards

Rupesh

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]]> Tue, 09 Sep 2008 23:43:37 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/job_posting_101111538t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/job_posting_101111538t.html questions, please let me know.

Lucy

*Postdoctoral Positions to Characterize Salicylic Acid Signaling Network
in Plants at Cornell Univ./BTI*



The plant hormone salicylic acid (SA) affects many diverse processes in
plant growth and development and plays important role(s) in response to
abiotic and biotic stresses. Its role(s) in disease resistance has been
the most extensively studied but is still only partially understood
(e.g. PNAS 2005, 102:1773; Science 2007, 318:113; Curr. Opin. Plant
Biol. 2008, 11:436). To obtain insights into how SA carries out its
varied functions, a large number of putative SA-binding proteins
(pSABPs) have recently been identified in Arabidopsis by affinity
chromatography. Investigators will use two powerful high throughput
screens, an Arabidopsis protein micro-array and a yeast three hybrid
system, to identify additional SABPs, as well as confirm the SA-binding
activity of the already identified pSABPs. These SABPs will be
characterized using biochemical, genetic, molecular, bioinformatic, and
biophysical (X-ray crystallography) techniques to decipher their
functions in growth and development and/or responses to abiotic or
biotic stresses (particularly disease resistance), and the effects of SA
binding on these functions. Based on current data and the results
obtained, an SA signaling network model(s) will be constructed, tested
by more detailed characterization of putative signaling or regulatory
modules, and refined in order to produce an exemplary model of the SA
signaling network of SABPs which may serve as a paradigm for other plant
hormones (and other small ligands). This multi-disciplinary project will
be supported by a 4-year NSF 2010 grant. Applicant should have a Ph.D.
in biochemistry, molecular biology, chemistry, genetics, or related
areas and must be strongly motivated and dedicated to doing basic
research. */Strong background in protein chemistry and enzymology is
preferred./*
*/ /*

Send CV and a cover letter detailing experience and _have_ three letters
of recommendation sent to: Dan Klessig, Boyce Thompson Institute for
Plant Research at Cornell University, Tower Road, Ithaca, NY 14853 or
email dfk8@cornell.edu
cornell.edu>.
Related website http://bti.cornell.edu/DanKlessig.php


Institute Description: BTI is a not-for-profit research institute
affiliated with Cornell University. Its research emphasis is on
fundamentally understanding the basic biology of plants, particularly as
this relates to signal transduction within a plant and between a plant
and its environment. BTI is an Equal Opportunity/Affirmative Action
Employer.


--
Lucy Pola, SPHR
Director of Human Resources
Boyce Thompson Institute
Tower Road
Ithaca, NY 14853

607-254-1239
lbp8@cornell.edu
http://bti.cornell.edu

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]]> Tue, 09 Sep 2008 12:27:00 PDT http://www.nnseek.com/e/bionet.genome.arabidopsis/bac_library_construction_101110770t.html http://www.nnseek.com/e/bionet.genome.arabidopsis/bac_library_construction_101110770t.html
I am a PhD student working in India . I have been attempting to construct a
High Molecular Weight (HMW) DNA library using
BAC but have been completely unsuccessful at producing clones. I know the
problem is with ligating the HMW DNA to the vector. I have already checked
the ligase and my vector and neither of those appears to be the problem. The
problem seems to be restricted to the HMW DNA.I am also worried about the
ligation reaction as I have already tried with different molar ratios as
advised in many papers but couldn't get any better result. Can you please
help me in this regard. Your help would be greatly appreciated.

Waiting for your kind reply,

Regards,

Sanjiban

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]]> Tue, 09 Sep 2008 07:19:15 PDT