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Author: Nate WaldronNate Waldron
Date: Jun 25, 2008 08:18
Hello, I found your posting about removing rubisco from plant extracts using
PEI and I was wondering if it would be possible to get the protocols you
used to develop the stock solution of PEI and the actual extraction
protocol. I have been having a very similar problem with a protein I've been
trying to extract/purify. My protein will only come out in alkaline pH's
with fairly high salt concentrations, which, as you know, also extracts a
whole lot of RubisCo. My protein is also very similar in size to Rubisco as
well which has made it harder to purify. Anyway, if you wouldn't mind
emailing a copy of the protocols I would really appreciate it. Thanks much.
-Nate Waldron
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Author: Venugopal KalavacharlaVenugopal Kalavacharla
Date: Jun 25, 2008 05:45
Post Doctoral Research Associate-Plant Molecular Genetics, Delaware State
University, Dover, DE
The College of Agriculture and Related Sciences at Delaware State
University is seeking qualified applicants for the position of Post
Doctoral Research Associate, Plant Molecular Genetics. Applicants must
have experience working with the genetics of plant host-pathogen
interactions, construction of genomic and cDNA libraries; RNA interference
is an added plus.
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Author: Eugenia RussinovaEugenia Russinova
Date: Jun 22, 2008 23:22
Dear colleagues,
Could you please post our ad on your mailing list (see the text below)?
With kind regards,
Jenny Russinova
CropDesign NV, located in Gent, Belgium is one of the worldwide leading
, inoovative biotech companies, focusing on the improvement of crops.
CropDesign has developed the high throughput phenotyping...
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Author: Kulbir SinghKulbir Singh
Date: Jun 20, 2008 12:06
Hello everybody,
I have a problem in Gus staining. The problem is that the gene i am
interested in, is expressed at low levels. So i dont see gus staining in the
tissue where i expect to see it until i have a line with multiple copies of
*Gene promoter:Gene:GUS* fusion construct. I have screened around 110 T2
lines to find around 7-8 homozygous lines in T3 generation with single locus
insertion. But none of them expresses gene at a level to see the Gus
staining in tissue i expect. Can anyone who had this problem suggest me what
i can do in my situation. I am trying to screen more T2 lines by
tranforming some plants again. But this will take few months to reach the T3
generation.
Thanks,
--
Kulbir Singh
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Author: Jane GlazebrookJane Glazebrook
Date: Jun 19, 2008 16:28
POST-DOCTORAL POSITION IN ARABIDOPSIS DISEASE RESISTANCE
Jane Glazebrook Laboratory
Department of Plant Biology
University of Minnesota
St. Paul, Minnesota, USA 55108
A post-doctoral scientist is needed to study genetics of
disease resistance in Arabidopsis thaliana. Part of the project involves
study of genes required for resistance to the fungal necrotroph Alternaria
brassicicola. Another part of the project involves investigation of the
effects of cell wall alterations on resistance to various pathogens. The
work provides opportunities to develop and enhance skills in genetics,
molecular biology, and computational biology. Interested scientists may
also participate in programs to develop teaching skills. The position is
available beginning September 1, 2008, and is funded for three years.
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Author: Peifen ZhangPeifen Zhang
Date: Jun 19, 2008 15:10
Dear Plant Researchers,
The Plant Metabolic Network (PMN) is pleased to announce the initial
launch of its official web site ( http://plantcyc.org) and the first
release of PlantCyc, a comprehensive biochemical pathway reference
database dedicated to the plant kingdom.
The PMN is an NSF-funded collaborative project among databases and
biochemists with a common goal to build a broad network of plant
metabolic pathway databases. The central database of PMN, PlantCyc...
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Author: Tobias BaskinTobias Baskin
Date: Jun 19, 2008 12:31
Postdoctoral Position
Auxin transport in arabidopsis roots
University of Massachusetts, Amherst, MA
Bard College at Simon's Rock, Great Barrington, MA
The labs of Eric Kramer (Bard College at Simon's Rock) and Tobias
Baskin (University of Massachusetts, Amherst) have a position for a
postdoctoral fellow. Kramer and Baskin have a joint, NSF-sponsored...
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Author: channa keshavachanna keshava
Date: Jun 19, 2008 00:58
I am a PhD student in Prof. Huelskamp lab, Cologne, Germany. I am in need of
Arabidopsis wildtype seeds of Nossen (No-0) ecotype. If anybody has please
send it to me soon.
Channa Keshavaiah
Botanical Institute-III
Gyrhofstrasse-15, 50931
University of Cologne, Cologne
Germany
On 6/18/08, arab-gen-request@ oat.bio.indiana.edu <
arab-gen-request@ oat.bio.indiana.edu> wrote: >
> Send Arab-gen mailing list submissions to
> arab-gen@ net.bio.net
>
> To subscribe or unsubscribe...
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Author: ZENG, QIN [AG/1005]ZENG, QIN [AG/1005]
Date: Jun 18, 2008 11:16
If you have answer for the following questions, I would really
appreciate it. What is the history of chloral hydrate being used as the
tissue clearing agent? What are the tissue clearing mechanisms for
chloral hydrate? Are there any other chemicals that have equivalent
functions?
Thanks,
Qin
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Qin Zeng, Ph.D
700 Chesterfield Village Parkway, GG5181-D
Chesterfield, MO 63017
(636)-737-6970
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Author: Nemo PeetersNemo Peeters
Date: Jun 18, 2008 03:41
19-months postdoc position, at LIPM, Toulouse, France
To study the mode of action of the F-box "GALA" type III effector
proteins of Ralstonia solanacearum on Arabidopsis.
Available from 1st September 2008 (starting no later than 1st January 2009)
Location:
The LIPM ( www.toulouse.inra.fr/centre/lipm), in Toulouse, France, is a
dynamic and international group of researchers studying plant-microbe
interactions. It provides an excellent scientific environment and
facilities to carry out this project.
The Position:
Together with the project leader, a PhD student and technical
assistance, the candidate will carry out Arabidopsis molecular genetics,
bacterial infection studies, protein biochemistry in order to identify
and characterize the targets of the GALA proteins injected by
R. solanacearum in plant cells.
Annual Gross salary of 25 611 EUR (expected Net salary around 1800
EUR/month including health and unemployment benefits).
Starting the 1^st of February 2008 or soon after.
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