i am working on tomato disease resistant gene screening i.e MAS( marker
assisted selection) . i have did the DNA isolation from
green house plant and open field plant. and complete the pcr for diffent
resistant gene. for near about 1500 sample the pcr is working fine got the
good amplification, but when i have again collected the samples after 30 day
the DNA Quality looks good on gel ,but not get amplified in pcr reaction. we
are tried the things but its not working. i would be thankful if some one
suggest me to over come the problem.

lookinforward for suggestion

With Regards

Rupesh

Please post this job to the list serve. Thank you. If you have any
questions, please let me know.

Lucy

*Postdoctoral Positions to Characterize Salicylic Acid Signaling Network
in Plants at Cornell Univ./BTI*

The plant hormone salicylic acid (SA) affects many diverse processes in
plant growth and development and plays important role(s) in response to
abiotic and biotic stresses. Its role(s) in disease resistance...

Dear Sir,

I am a PhD student working in India . I have been attempting to construct a
High Molecular Weight (HMW) DNA library using
BAC but have been completely unsuccessful at producing clones. I know the
problem is with ligating the HMW DNA to the vector. I have already checked
the ligase and my vector and neither of those appears to be the problem. The
problem seems to be restricted to the HMW DNA.I am also worried about the
ligation reaction as I have already tried with different molar ratios as
advised in many papers but couldn't get any better result. Can you please
help me in this regard. Your help would be greatly appreciated.

Waiting for your kind reply,

Regards,

Sanjiban