The larval tissue is sticky if u do not add the detergent -and forms clumps
or stick to poor quality eppendorfs ( silocanised brands are good )
usally we use PTX ( 0.1%% triton X 100 ) as blocking solution at 4 degree
storage.
during changes use the tips rinsed once in this solution while transferring
the tissue ( it is better use 1000ul tip or cut tips for p200)
but i do not advice storing the samples in this solution for long time .,
they damage the tissue

another thing is gut is the soruce of lot of bacterial contamination !!! so
need to take care of theat as well !! i hope u can also use the sodium azide
in solutions ( hezardous to humans too . so caution while handling!!)

another reason could be that the tissue is not properly fixed !! check the
fixative parafomaldehyde ( which may be old and not working ???)

i hope this would help to sortout the problem

with best reagards
Gurudatta

On Dec 28, 2007 12:03 PM, oat.bio.indiana.edu> wrote:

HI. i am working with gut of drosophila. i would like
to know about the way to handle and store the fixed
guts. i am storing the fixed guts (approx 10-15 in
number) in an eppendorf tube with PBS. the problem i
face is that while i pipette out the sample, it gets
sheared (as i need the entire gut for my investigation
purpose). also sugggest a way to section the gut (to
make a vertical tear in its surface) for antibody
staining purpose. as i am totally new to the area, i
would be greatful if you can guide me with the whole
thing (also as soon as possible),

thank you
gayatri

__________________________________________________________
Sent from Yahoo! Mail - a smarter inbox http://uk.mail.yahoo.com

Global Change Biology

The Department of Biology at the University of Massachusetts at Amherst is seeking to fill three tenure-track faculty positions at the Assistant professor level:

One position is broadly defined as the area of Ecological Physiology. We are looking for a researcher whose work is field-based and integrative, and are particularly interested in researchers using genetic and hormonal approaches within an ecological context. Organismal focus (animal or plant) is open.

The second position is in the area of Endocrine Disruption. We are seeking a researcher whose interest is in effects of environmental contaminants on endocrine physiology. We are particularly interested in researchers examining the physiological mechanisms underlying endocrine disruption.

The third position is in the area of Plant Metabolism. We are seeking a researcher who uses systems biology and/or functional genomic approaches to understanding plant metabolism. The area of research should be relevant to the use of plants for bioenergy, for example, carbon metabolism or biopolymer production by plants.

The researchers would be expected to participate in a broad multi-disciplinary initiative in Global Change Biology within the Department of Biology. This initiative bridges a group of faculty who use multiple levels of analysis to understand how rapid environmental changes are impacting populations and individual organisms, including: loss of biodiversity, rapid evolution, disruption of physiology, reduced agricultural outputs, and evolution of new pathogens. Postdoctoral experience required.

Applications, which should include CV, statements of research interest and teaching philosophy, and the names, addresses and e-mails of at least 3 references, should be sent to: Biology Search c/o Ms. Karen Nelson, Biology Department, University of Massachusetts, Amherst, MA 01003. It is very important that you reference the position number to which you are applying. Positions to be filled contingent upon funding. The position numbers are as follows:

Ecological Physiology R32351
Endocrine Disruption R32352
Plant Metabolism R32353

Evaluation of applications will begin on December 10, 2007 and continue until the positions are filled.

The University of Massachusetts is an Affirmative Action Equal Opportunity Employer. Women and members of minority groups are encouraged to apply. The Biology Department is aggressive in its efforts to hire candidates who will enhance the diversity and general balance of the faculty and the sciences.

Hi everyone,

I'm trying to make a transgene with a flip-out cassette (FRT-stop-
FRT). Does anyone happen to have a plasmid that contains this
fragment and can send me a small aliquot? Many thanks!

Adelaine

Dear All,

Does anybody have a pCaSpeR yellow + vector instead of white+
to establish a transgenic flies?
If you have it, please let me know.

Thanks

Ritsuko SUYAMA

*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+
Ritsuko Suyama, phD.
Ephrussi /Akhtar Group,
Developmental Biology/Gene Expression
EMBL
Meyerhofstrasse 1,
D-69117 Heidelberg
Germany

*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+

Dear All,

Does anybody have a pCasper yellow + vector instead of white+
to establish a transgenic flies?
If you have it, please let me know.

Thanks

Ritsuko SUYAMA

*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+
Ritsuko Suyama, phD.
Ephrussi /Akhtar Group,
Developmental Biology/Gene Expression
EMBL
Meyerhofstrasse 1,
D-69117 Heidelberg
Germany

*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+

Hi all,
Does anybody know how to get the Sarstedt cryovials? They were available till some time back but
now I cannot find them through any vendor. I would appreciate any info.

Thanks,
Payal Ray

Our solution was to wait till the first flies start to emerge from the
pupae, and wash those using a chloride solution. We try to synchronize
the flies within a vial as much as possible, in order to reduce loss of
non-pupated larvae.

Hope this helps.

Kim

azza sellami wrote:

Hi, you mean "eradicate"?
Pass the flies in vials twice a day for several days and collect embryos
from a several hours incubation, wash them in chlorax, then examine them
for mites under a microscope, transfer (a dozen of eggs are enough) to a
flesh vial and amplify.

Good luck,
Maki

Maki Asano, M.D., Ph.D.
Assistant Research Professor

Rm389 CARL Bldg P.O. Box 3657
Department of Molecular Genetics and Microbiology
Duke University Medical Center
Research Dr. Durham,
NC27710, USA

E-mail address: asano@duke.edu
Phone: 1-919-684-2392
Fax: 1-919-681-8984
http://mgm.duke.edu/faculty/asano/index.htm

On 12/3/07 10:31 AM, "azza sellami" wrote:

Dr. Erickson lab protocol for get rid of mites (by dechorination):

1) Let flies to lay eggs onto usual vial for overnight or 7 hours.
2) Add 5 ml 50%% bleach for 3 min
3) pour out bleach (accurately). Check that eggs are on the surface of
the vial.
4) add 10 ml PBS
5) pour out PBS (accurately). Check that eggs are on the surface of the
vial
6) add 10 ml PBS
7) pour out PBS (accurately). Check that eggs are on the surface of the
vial
8) Let eggs to hatch 37oC 10 days.

Protocol#2 (less efficient)

1) Transform flies every day onto new vial. Repeat 4 times. Grow flies
from the 4th vial.
2) Repeat the same with the progeny from the 4th vial.