http://www.pnas.org/content/103/47/17813
will be useful to modify the construct described in the above.
sd

Does anybody know if there are Drosophila integration vectors available
with two promoters that can be used to clone and express two different
proteins from the same construct after single integration event?

Thanks in advance,

Priit Joers

Hello to all Drosophila injecting people,

All of you may know how essential it is to have a good needle!
I'm currently using pre pulled capillaries from Eppendorf (Femtotip I).
But they have a big variance in length, diameter and they are not
open (as Eppendorf claim in their description)
Is anyone of you using the Femtotips? Or what capillaries do you use?
It would be great if you can share your experience with me.
Maybe there is an other company with better pre pulled capillaries
where I can order from Germany?

Many thanks,
Sandra

Drosophila genomicists,

You may find useful material in this new orthology comparison of Arthropod
genes at http://insects.eugenes.org/arthropods/

euGenes/Arthropods contains computed protein orthologs among 13 species:
3 mosquitoes, 3 drosophila, 2 hymenoptera, 3 other insects, a crustacean
and an arachnid.

These can be searched by gene names, descriptions and IDs. You can also
select gene groups by presence or absence in a species. Find genes also
by BLAST, including PSI-BLAST, of the ortholog groups.

You can for instance find the 7 cases in 27497 groups where the other
insects, including Dros. pse. and Dros. moj., have a gene that Dros. mel.
is apparently missing (I think I've found most of these in Dmel's genome :)

You can also find these Arthropod gene pages via Google searches,
Google: daphnia drosophila ixodes gene homeobox
Google: drosophila aedes aphid gene ribosomal protein L13a

especially if you add these newer genome organisms others do not yet report.
Google searches are not as certain to pick up all the
arthropod gene groups as the search at euGenes/arthropods.

For small batches, I find using *any* pump to be more trouble than
it is worth: I pour them by hand, both the thick and the thin. I
use a one liter plastic beaker with a sharp lip, never try to pour
from more than ca 300 ml at a time, and arrange the setup so that I
never have to pour across more than 4 vials in a tray (my trays hold
9 rows, so I pour the right-side 5 rows then turn the tray around to
pour the remaining 4). With a little practice one gets pretty good
at uniformity but it's never perfect, so I set the too-little ones
aside and use them for storing virgins and fertility tests. Takes
me 5 minutes per 100 vials: and no pump to clean up afterwards!

And I cook all my fly food over a gas hob -- pan just needs to be
large enough so that when the food boils up it doesn't boil over,
nothing special there.

Hope this helps, and feel free to contact me directly with any
further questions!

Cheers,
Adelaide Carpenter

hrundle@uottawa.ca wrote Mon, 25 Aug 2008 11:14:47 -0700 (PDT)

watson marlow 520 series pumps will work the best. www.watsonmarlow.com

?Ask me about Miele Glassware Washers.

Best Regards,

Scott Saveleski
Atlantic Technology Group, Inc.
1906 North Hamilton Street, Suite G
Richmond, Va. 23230
1-800-359-5153 x2
410-371-8740 cell

-----Original Message-----

Sent: Tue, 26 Aug 2008 1:04 pm
Subject: Dros Digest, Vol 40, Issue 4

Send Dros mailing list submissions to
dros@net.bio.net

Hello Flyland,

Well it's time to buy a needle puller. I have only ever used the
Sutter horizontal P-97 with two stage pulling, and that machine costs
about $9000 all told. I am very cautious about buying a cheaper
machine having never had any experience with any other make, but am
balking at the $9000 price tag of the Sutter.

Please send me your feedback about needle pullers you have known and
loved. The machines, I mean.

Many thanks,

Sandra

Dept/School Human Genetics Unit, Medical Research Council
Project Title Drosophila Screen for Regulators of RNA editing
Project Supervisor(s) Dr L Keegan, Dr M O’Connell
Application Deadline 22 August 2008

The MRC Human Genetics Unit is offering a three year PhD studentship
funded by the Scottish Motor Neurone Disease Association working under
the supervision of Dr Liam Keegan. This excellent opportunity would suit
a student finishing an MSc project on Drosophila or a student graduating
with a good training in Genetics and Developmental Biology and some
experience of working with Drosophila or another efficient model organism.

The project is based in lab studying RNA editing. This is a step in RNA
processing that affects the expression levels and the amino acid
sequences of vertebrate glutamate receptors. Loss of RNA editing has
been reported in motor neurones of ALS patients (Kawahara (2004) Nature
427, 801). and following ischaemia in central neurons (Peng (2006)
Neuron 49, 719). Glutamate is the major excitatory neurotransmitter in
vertebrate CNS and loss of the edited forms of vertebrate glutamate
receptors is expected to promote neuron death by allowing excessive
calcium influx.

2008-08-07

Do anybody have experience on making FRT-directed deletions using Exelixis stocks described in Park et al 2004 Nat.Genetics paper? I did one WH minus - RB plus deletion, but failed to find any correct deletion in 96 lines via PCR. My PCR was using similar primers described in Park et al paper. I did 20ul rxn with Takara LA Taq. Annealing temperature touchdown from 68c, 65c, 62c, 59c (each 2 cycle) then 56c 25 cycles. I used 1 min annealing and 72c, 12min extension. The product should be ~7.3kb according to the paper, but I failed to get any band in between primer and the well. However there were thick bands in the well.

Could anybody help me to figure out what is the problem? Thanks a lot!

Best,
Yi

--
No fate but what we make.

Zhang Yi
Lab of Neuroscience, B109
National Institute of Biological Sciences,
7 Kexueyuan Rd. ZGC Life Sciences Park,
Beijing 102206
China, People's Republic of
http://algebra.yculblog.com

Electrophysiology Postdoctoral Position Available
Northwestern University

The Allada laboratory is seeking highly motivated individuals with
prior experience with electrophysiology for a postdoctoral position.
This position is available immediately.

Our research is focused on the regulation of circadian and sleep
behavior using the fruit fly Drosophila and incorporates patch clamp
electrophysiology along with a variety of other approaches including
biochemistry, molecular biology, genetics, cell culture, anatomy, and
behavior (see Lear et al., Neuron, 2005, Pitman et al., Nature,
2006). This position offers the opportunity to integrate
electrophysiological, with molecular genetic and behavioral
approaches. See also
http://www.northwestern.edu/neurobiology/faculty/allada.html