DK wrote: In article <6dljquF36jrlU1@mid.individual.net>, Kyle Legate <legatek@hotmail.com> wrote: Lara wrote: Dear DK and Han, thanks so much for your helpful feedback. Indeed, now I understand it is not a waste of either Trypsin or EDTA. With responses like this newsgroups get another dimension in science. Thank a lot again! It most certainly is a waste of

Dear DK and Han, thanks so much for your helpful feedback. Indeed, now I understand it is not a waste of either Trypsin or EDTA. With responses like this newsgroups get another dimension in science. Thank a lot again! Yours, Lara On Jul 8, 5:24 pm, Lara <eudo...@googlemail.com> wrote: Hello there folks, I use for some endothelial cells trpsin/edta and according to

Hello there folks, I use for some endothelial cells trpsin/edta and according to the protocol I have to pipette in 5 ml trypsin/edta and then immediately remove 4.5 ml and leave the rest for a couple of minutes. Does anyone know why? Isn't this simply a waste of trypsin? Furthermore, is there any place where I can find detailed info about trypsin in particular? Thanks for any info. Cheers

> Washing with PBS is preferable because it more effectively removes the inhibitors (as you don't leave any behind in the flask when you aspirate all the PBS), and washing with PBS first removes dead cells and debris much more effectively. I am not denying any of what has been written so far but you have to apologize if I might ask you what constitutes chemically the fact that it is more

Am 23.06.2008, 10:03 Uhr, schrieb Behdani <behdani@pasteur.ac.ir>: Hello All I have to isolate cytoplasmic membrane receptor from 293 cell line (Human Kidney) to immunize rabbit for polyclonal antibody production. I am looking for a protocol (lysis buffer and other steps) which contains reagents which save native form of membrane proteins. Could somebody The following recipe