In our (Reading, West) LiDL today I saw a pressure washer, of the well-known Neverheardofem brand (actually it says 'LAVOR' on it), which for £110 boasts: Superwash 160 Pressure Washer * Max. pressure (bar): 160 * Flow rate (l/hr): 530 * Auto stop/start * Accessories include: * - Spray gun with pressure gauge * - High pressure hose, length (m): 10 * - Integrated

It is really depressing when you donot get the desired results. If the plasmid is good sometime you loose sites that are involved in restriction followed by ligation, others remain unaffected. It also happens if the plasmid is isolated using phenol chloroform extraction using old preparation of phenol ( gets oxidized with time and makes restriction sites resistant to cut). I suggest go back to

-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dick Cheney Needs a Good Scrubbing Via NY Transfer News Collective * All the News that Doesn't Fit The New York Times - Sep 13, 2006 http://select.nytimes.com/2006/09/13/opinion/13dowd.html?n=Top%%2fOpinion%%2fEditorials%%20and%%20Op%%2dEd%%2fOp%%2dEd%%2fColumnists%%2fMaureen%%20Dowd Vice Must Wash Hands Before Returning to Work By

* days until America elects a new congress* "Morton Davis" <antikerry@go.com> wrote in message news:pP4Og.186612$1i1.69433@attbi_s72... "Lefty" <someone@sbcglobal.net> wrote in message news:TZ2Og.1535$7I1.65@newssvr27.news.prodigy.net... "Sid9" <sid9@bellsouth.net> wrote in message news:Wl0Og.6068$C26.860@bignews6.bellsouth.net... September 13, 2006 Op-Ed

"Lefty" <someone@sbcglobal.net> wrote in message news:TZ2Og.1535$7I1.65@newssvr27.news.prodigy.net... "Sid9" <sid9@bellsouth.net> wrote in message news:Wl0Og.6068$C26.860@bignews6.bellsouth.net... September 13, 2006 Op-Ed Columnist Vice Must Wash Hands Before Returning to Work By MAUREEN DOWD Washington I called Tim Russert to ask if