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One thing more- retransform. Pick up 5-6 single clones (Blue clnes on X-Gal/IPTG) culture individually and isolate plasmid from each culture. Compare restriction and select the best one. On 7/3/08, methods-request@oat.bio.indiana.edu <methods-request@oat.bio.indiana.edu> wrote: > Send Methods mailing list submissions to > methods@net.bio.net > > To subscribe or unsubscribe via the World     

Group: comp.softsys.matlab · Group Profile · Search for Jcamp in comp.softsys.matlab
Author: KMP
Date: Apr 25, 2007 15:58

It is really depressing when you donot get the desired results. If the plasmid is good sometime you loose sites that are involved in restriction followed by ligation, others remain unaffected. It also happens if the plasmid is isolated using phenol chloroform extraction using old preparation of phenol ( gets oxidized with time and makes restriction sites resistant to cut). I suggest go back to
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Dear fellow workers in the mines of science, I have been tryingto work with the pCambia1201 plasmid for the lastseveral weeks, but ithas been driving my lab a bit crazy. My problemis that it does not wantto digest with most endonucleases we've tried.I simply can not use theplasmid if I can't cut it, so I have aproblem. It was suggested to meto try transferring the plasmid to DH5cells, which I did     

Group: bionet.molbio.methdsreagnts · Group Profile · Search for Jcamp in bionet.molbio.methdsreagnts
Author: Virash Gupta
Date: Jul 5, 2008 05:23

Dear Colleagues, I have several questions about practical plant RNAi to which I have been unable to find the answers in the literature. I suspect that these questions are mostly naive, but I since none of my colleagues here on my own campus work with plant RNAi, I thought I may be able to find the answers out here. First, are there any good practical guides or reviews out there on plant
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Has anyone out there used Nam-Hai Chua's Dex-inducible plant expression vector (pTA7001) with Medicago truncatula? He does not seem to know whether or not it works with the species. I just want to be reassured before I go to the great trouble of obtaining and implementing this particular plasmid. Thanks, Jim James J. Campanella, Associate Professor, Department of Biology and Molecular     

Group: bionet.molbio.methdsreagnts · Group Profile · Search for Jcamp in bionet.molbio.methdsreagnts
Author: Virash Gupta
Date: Jul 5, 2008 05:20

Thanks to liberalism.. "Eggs Ratzinger Arnold" <maxpontifex@yahoo.it> wrote in message news:1178236465.309048.131520@o5g2000hsb.googlegroups.com... Jesus Camp God Help Us All! By Alton Raines altonraines(at)minister.com May 3, 2007 http://www.rense.com/general76/jcamp.htm Indrid Cold wrote: Whitley's Starboard Brainfart? "The oldest and strongest emotion of
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"Cthulhu: Who will be eaten FIRST?!" http://maxpontifex.blogspot.com/ Eggs Ratzinger Arnold wrote: > > Jesus Camp > God Help Us All! > By Alton Raines > altonraines(at)minister.com > May 3, 2007 > http://www.rense.com/general76/jcamp.htm > > Indrid Cold wrote: > > > Whitley's Starboard Brainfart? > > > > "The oldest and strongest emotion of mankind is fear, > > and the oldest and     

Group: bionet.molbio.methdsreagnts · Group Profile · Search for Jcamp in bionet.molbio.methdsreagnts
Author: James J. Campanella
Date: Jul 1, 2008 16:34

"Cthulhu: Who will be eaten FIRST?!" http://maxpontifex.blogspot.com/ Eggs Ratzinger Arnold wrote: Jesus Camp God Help Us All! By Alton Raines altonraines(at)minister.com May 3, 2007 http://www.rense.com/general76/jcamp.htm Indrid Cold wrote: Whitley's Starboard Brainfart? "The oldest and strongest emotion of mankind is fear, and the oldest and
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Jesus Camp God Help Us All! By Alton Raines altonraines(at)minister.com May 3, 2007 http://www.rense.com/general76/jcamp.htm Indrid Cold wrote: > Whitley's Starboard Brainfart? > > "The oldest and strongest emotion of mankind is fear, > and the oldest and strongest kind of fear is fear of > the unknown." > - H. P. Lovecraft, "Supernatural Horror in Literature" > > "Whitley Strieber"     

Group: bionet.molbio.methdsreagnts · Group Profile · Search for Jcamp in bionet.molbio.methdsreagnts
Author: Dr. James J. Campanella
Date: Aug 29, 2007 06:55

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Group: bionet.molbio.methdsreagnts · Group Profile · Search for Jcamp in bionet.molbio.methdsreagnts
Author: Dr. James J. Campanella
Date: Aug 15, 2007 07:16

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Group: alt.alien.visitors · Group Profile · Search for Jcamp in alt.alien.visitors
Author: Chairman Mao says:
Date: May 3, 2007 22:16

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Group: alt.fan.rawilson · Group Profile · Search for Jcamp in alt.fan.rawilson
Author: Jupiter
Date: May 3, 2007 17:40

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Group: sci.physics · Group Profile · Search for Jcamp in sci.physics
Author: Jupiter
Date: May 3, 2007 17:40

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Group: alt.alien.visitors · Group Profile · Search for Jcamp in alt.alien.visitors
Author: Eggs Ratzinger Arnold
Date: May 3, 2007 16:54

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